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Total RNA extraction and RT-qPCR Total RNA was extracted from tissues and cultured cells using the RNAiso Plus reagent (Takara Bio, Tokyo, Japan) in accordance with the manufacturers protocol

Sirtuin deacetylases, also known as class III KDACs, are localized in various cellular compartments, including the nucleus (SIRT1 and SIRT6), nucleolus (SIRT7), cytoplasm (SIRT2), and mitochondria (SIRT3, SIRT4, and SIRT5) [61]
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3 Proteins, gut microbiota, and depression 3.1 Association between protein intake and depression Adequate protein intake is essential for human growth, development, and health maintenance (12), and it is also associated with the prevalence of depression (2730)

doi: 10.5772/intechopen.91827 90

doi: 10.1016/j.brainres.2008.11.053
