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The Sirtuin family is a nicotinamide adenine dinucleotide (NAD + )-dependent histone deacetylase with seven members (SIRT1-7), is involved in many crucial biological processes, including apoptosis, endocrine signaling, glucose homeostasis, aging, and longevity [108]

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Tissue was then incubated in a 1:2:8 (v/v/v) solution of DAPI, 0.0004% Fluoro-Jade C and distilled water for ten minutes, taking precaution to protect the solution from light

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