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In this procedure, Cu was first reacted with albumin to form a Cu-albumin complex, followed by a microbubble formation through ultrasonic vibration

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(For reviews, see Anders, 2004, Anders, 2008, Cooper, 1998, Cooper and Pinto, 2008, Cooper et al., 2011, Dekant, 2003, Dekant et al., 1994.) The reactive fragments generated from haloalkene cysteine S -conjugates by the action of cysteine S -conjugate -lyases are especially cytotoxic in experimental animals to the proximal renal tubules, in particular to the S 3 ( pars recta ) region and, to a lesser extent, the S 1 and S 2 ( pars convoluta ) region (e.g., Jones et al., 1988) and to isolated human proximal tubules (Chen et al., 1990)