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Nat Med (2014) 20:63341

50 mg per day: Higher doses are less common and should only be considered under strict medical supervision

15 However, there are weak recommendations against using melatonin for the treatment of insomnia in American and European guidelines

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Immunochemistry Cells were fixed with 4% paraformaldehyde for 20 min at 4 C, permeabilized with 0.125% Triton X-100 in PBS and blocked with True Black (Biotium) according to the manufacturers directions and then 10% normal goat serum (NGS) for 1 h

1997 Aug;11(4):787-91