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There were no significant differences observed between each study group regarding age, disease history, previously used medications, prostate and testis volume, and initial hormone levels

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Brains were homogenized using lysis buffer for whole protein and mitochondrial protein extraction as previously described (Nguyen et al., Novex 4%12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes

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Thermo Fisher Scientific), and CRISPRMAX Cas9 Plus Reagent (CMAX00015