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Neurobiol Stress

Pelleted Sf9 cells were lysed in lysis buffer containing 20 mM Tris pH7.5, 2.0 mg ml 1 iodoacetamide, 0.2 g ml 1 leupeptin (Sigma), 100 g ml 1 benzamidine (Sigma), and 0.5 M finasteride by stirring at 4 C for 1 h

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Formalin-fixed tissue was stained with eosin using previously described procedures ( Estimation of Oxidative Stress The concentration of MDA in hippocampal homogenates as an index of lipid peroxidation was determined according to a previously described method (Waterborg and Matthews, 1984)

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it is activated by various physiological stimuli known to produce ROS