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Particularly in liver cirrhosis associated with thalassemia major, which concomitantly can impair pituitary GH secretion, there is no secondary upregulation of local IGF-I and linear growth failure is common (95)

To construct the shRNA knockdown vector pLKO.1-GST DAF , we inserted the GST-EGFP-GPI DAF coding region into the pLKO.1 puro plasmid in place of the puromycin resistance gene

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