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Method Description Steps Manual Freezing A step-by-step method involving gradual temperature reduction to ensure cell viability

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an additional six common symptoms such as changes in sexual desire or capacity, loss of or change in smell or taste, thirst, chronic cough, chest pain, and abnormal movements were included

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For examination of cytokines and cellular proliferation, the following antibodies were used: TNF (MAb11, 1:200), IFN (4S.B3, 1:200) and Ki-67 (Ki-67, 1:400)

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