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Standards were prepared in matching dilutions of the lysis/extraction buffer in KPE buffer

10.1016/j.neuroscience.2014.05.051 157 RossignolJ.CozziB.LiebaertF.HattonS.ViallardM

The above results show that GPX4 inhibitors, such as RSL3, are senolytic

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The BIOXYTECH assay is based on the SODmediated increase in the rate of autoxidation of 5, 6, 6a, 11b-tetrahydro-3, 9, 10-trihydroxybenzo[c]fluorene R1 in aqueous alkaline solution to yield a chromophore with maximum absorbance at 525 nm