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Cells were grown in YPD (1% yeast extract, 2% peptone and 2% glucose), YPG (same as YPD, with 2% glycerol instead of glucose), or minimal media (SD) (0.67% yeast nitrogen base w/o amino acids, 2% glucose), or minimal media glycerol (SG) (same as SD with 2% glycerol instead of glucose) with aminoacid supplements as appropriate, at 30C

Did you know that an estimated 40% of the population is deficient in vitamin B12