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Description
Allergy 44 , 12001203 (2014)

In younger individuals refractive error, myopia or hyperopia is often caused by abnormalities in corneal curvature or the length of the globe, but rarely by defects in the curvature or refractive index of the lens
E.Carrillo-CrdovaD.Oria-HernndezJ.Snchez-ValleV.Ponciano-RodrguezG.Ramrez-JaramilloM.et al (2015)

Clin Biochem 26(5):351357

The staining solution was then removed and the cells were washed three times with PBS

This is a normal physiological process
