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For shoot WC measurement, the shoots were excised, and their FW was immediately recorded

Abbreviations 4-PB: 4-phenylbutyrate A1AT: Alpha 1-antitrypsin ABC: ATP-binding cassette AFP: Alpha-fetoprotein ASBT: Apical sodium dependent bile acid transporter BAs: Bile acids BSEP: Bile salt export pump CA: Cholic acid CDCD: Chenodeoxycholic acid CYP: Cytochrome P450 DCA: Deoxycholic acid DCDC2: Doublecortin domain containing 2 FGFR4: fibroblast growth factor receptor 4 FXR: farnesoid X receptor G(T)CA: Glyco- or tauro-cholic acid G(T)CDCA: Glyco- or tauro-chenodeoxy cholic acid GGT: Gamma glutamyl transpeptidase HCC: Hepatocellular carcinoma IEBAM: Inborn error of bile acid metabolism LCA: Lithocholic acid MDR2/3: Multidrug resistance protein 2/3 MRP2/3/4: Multidrug resistance-associated protein 2/3/4 MYO5B: Myosin VB NASH: Nonalcoholic steatohepatitis NH: Neonatal hepatitis NICCD: Neonatal cholestasis caused by citrin deficiency NTCP: Sodium/taurocholate co-transporting polypeptide OATP1B1/3: Organic-anion-transporting polypeptide 1B1 and 1B3 OST/: Organic solute transporter-/ PC: Phosphatidylcholine PFIC: Progressive familial intrahepatic cholestasis PS: Phosphatidylserine RXR: Retinoid X receptor SERPINA1: Serpin family A member 1 SHP: Small heterodimer partner TFAM: Mitochondrial transcription factor A TGR5: Takeda G protein-coupled receptor 5 (G protein-coupled bile acid receptor 1 , GPBAR1) TJP2: Tight junction protein 2 UDCA: Ursodeoxycholic acid UGT1A1: UDP-glucuronosyltransferase 1A1 References Esteller A

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The NNMT inhibitor works bottom-up at the cellular level by addressing the enzymatic dysfunction within adipocytes themselves

The plates were then washed with 1 PBS, and cells were lysed with 50 l BioSpyder 1 lysis buffer for 15 min at RT

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