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Ritzel et al., 2016

This process obtained a 5-mutation variant (H21A, Y56T, V165I, Q166L, L211V, cpSFVenus numbering) that, when introduced in our previous variants, blue-shifted the maximum excitation wavelengths (400nm vs

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3B) [76], with polyethylenimine (PEI) being the best permeabilizer

However, there are also independent mechanisms between CR and IF