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Nose to brain delivery of donepezil through surface modified NLCS: formulation development, optimization, and brain targeting study

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Each animal was inoculated by subcutaneous injection of 2 10 6 HT-29 cells mixed with 1 10 6 freshly isolated human PBMCs, cells were mixed 1:1 v/v with LDEV-free extracellular matrix protein Geltrex (Gibco, # A1413201) and inoculated in the right flank under anaesthesia

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