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The immune system shifts dramatically toward Th2 dominance, flooding the skin with interleukin-4 (IL-4), interleukin-13 (IL-13), and interleukin-31 (IL-31)

Crystals were transferred and soaked in 2 L droplets of crystallization solution for 5 min to ensure sufficient incorporation of cryo-protectant (20% (w/v) PEG 3,350), and subsequently flash frozen in liquid nitrogen for storage

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