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This gap stems from methodological limitations: conventional cell cultures cannot sustain long-term exposure assessments, while animal models inadequately predict human immune responses or organ-specific damage [293]
Metabolite extraction and LC-MS/MS analysis To measure the uptake of -Glu-met peptide by SEO1, seo1 dug3-2 ecm38 (AB6601) cells with SEO1 overexpression and empty vector (control) were grown in SD without uracil medium overnight and reinoculated in SD without uracil and methionine medium at 0.02 OD 600 for 4-5 hours

doi: 10.1002/ijc.21191

216 Furthermore, a randomized, double-blind, placebo-controlled trial in 65 hemodialysis patients reported that taking 1,200 mg, but not 600 mg, of CoQ10 daily for four months decreased oxidative stress (as indicated by a decrease in F2-isoprostanes)