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Binding buffer Your choice of binding buffer will influence the binding capacity of the beads, so if you are capturing substantially less than the reported binding capacity of your beads, and your protein isnt really big, then it is worth considering if your binding buffer is limiting the capture efficiency of the beads

Among bioprocessors, attitudes toward affinity purification range from a desire to move beyond old specificity/yield trade-offs to a willingness to explore new polyhistidine technology spin-offs, including systems for real-time detection.

Store at room temperature between 1 and 30C

Our in silico analysis indicates homology of putatively active SbGLYI, SbGLYII and SbDLDH proteins to several functionally characterised glyoxalases and D-LDHs from Arabidopsis and rice