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Description
Thermal cycling consisted of initial activation (95C for 7 min) followed by 35 cycles of denaturation (95C, 30 s), annealing (56C, 30 s), and extension (72C, 30 s) and a final extention at 72C for 7 min
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Others stagger them for extended coverage
T.MinhC
Blood sample was drawn from the subject and placed in EDTA tube (Vacuette, Greiner Bio-One, Kremsmnster, Austria)
10.1016/j.ccl.2020.02.001 27 CebanF.LingS.LuiL
