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The study conducted a series of experiments to determine the optimal concentration of DTNB for the assay
Its effects on neurotransmitter systems may modulate pain perception

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[DOI] [PubMed] [Google Scholar] Vali S, Mythri RB, Jagatha B, Padiadpu J, Ramanujan KS, Andersen JK, Gorin F, Bharath MM

Usually, the N-terminal amino acid is cleaved
Dokholyan, Reference Dokholyan2016
