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Left gel shows very little GST-fusion protein in the flow-through and a lot in the elution indicating that sufficient glutathione agarose resin was used in this purification

Furthermore, 16S rRNA sequencing revealed a rebalancing of the gut microbiota, characterized by a reduced Firmicutes/Bacteroidota ratio, enrichment of beneficial taxa like Bacteroidota and Alloprevotella, suppression of pathobionts such as Desulfovibrionales, and partial restoration of microbial diversity

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