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When performed in vivo (Committee et al
Individual factors, including the severity of underlying metabolic dysfunction, lifestyle modification sustainability, and weight loss magnitude, influence whether long term treatment remains necessary
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After rinsing with PBS, all sections were incubated at room temperature for 2 h with IgG goat anti-rabbit (Thermo Fisher Scientific, Alexa Fluor 488) diluted 1:200 in 0.01 M PB containing 1% BSA and 0.3% Triton-X100
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