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GSH conjugation to the enolised ketone intermediates of ecosanoids could protect cells from their toxic actions

Roles of the pyruvate kinase isoform M2 in promoting cancer in manners unrelated to catalysis are reported elsewhere 132,133,134,135,136

Then we highlight the OSF pathogenesis with special emphasis on the role of TGF-b, epithelial-mesenchymal transition, and other processes such as dysregulation of collagen metabolism and angiogenesis

Biliary tree fragments and associated stroma were dissociated into single cells using 7x TrypLE (Gibco), incubated in 2% FCS with antibodies anti-EpCAM/CD326 (clone G8.8, APC, eBioscience, 6:100), CD45 (clone 30-F11, PE, BioLegend, 1:50), CD31 (clone 390, PE, BioLegend, 1:50), TER-119 (clone TER-119, PE, BioLegend, 1:50) and subjected to fluorescence-activated cell sorting (FACS) based on size and granularity and singlets using a BD Influx Cell Sorter