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The SPHK1 gene encodes a sphingosine kinase which is responsible for the synthesis of the bioactive lipid, sphingosine-1-phosphate, S1P

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Pi, inorganic phosphate

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Sodium hyaluronate aids with this too, thanks to its moisture-binding properties

The protein was separated by SDS-PAGE, then transferred to a PVDF membrane, and sealed with 5% BSA for 1 h2 h at room temperature