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For example, the novel Penicillium roqueforti strain could produce both tannase and GA simultaneously ( Aspergillus awamori strain BTMFW032, under deep fermentation conditions, was able to simultaneously produce GA and tannase, resulting in a 15-fold increase in the yields of both tannase and GA ( Rhodotorula pilimanae A45.2 and Bacillus spheroides have also been noted for their abilities to co-produce GA and tannase, with the latter achieving high efficiency in converting tannic acid to GA (90.80% crystallization), making it the most potent bacterial tannase producer for GA synthesis (Kanpiengjai et al., 2020

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Later, in the 1980s, the development of high-pressure liquid chromatography methods allowed for rapid determination of homocysteine concentrations in 1000s of samples, thus paving the way for epidemiological studies that revealed significant associations between relatively small elevations of homocysteine in blood and cardiovascular, cerebrovascular, and peripheral vascular diseases (7, 46)