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To monitor synaptogenesis in vitro , primary neurons were cultured at low density for 14 and 21 days (DIV14 and DIV21)

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The DCFDA-derived fluorescence was detected in the FL-1 channel, with excitation set at 488 nm and emission collected at 530 nm

[DOI] [PubMed] [Google Scholar] [54].Kislin M, Sword J, Fomitcheva IV, Croom D, Pryazhnikov E, Lihavainen E, et al

Considering that carotid intima-media thickness (CIMT) is a critical marker of early atherosclerotic lesions, the positive correlation between elevated levels of BCAAs and increased CIMT suggests a potential role for BCAAs in the early development of atherosclerosis (53)