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With the aid of X-ray crystallography and site-directed mutagenesis techniques, the roles of GST active site amino acid residues were identified and evaluated [62, 81]

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70 At 3 weeks, the pain scores of both groups had significantly decreased from 5.6 and 5.1, respectively, to 0.9 in both groups, with no difference between groups

Batch culture of engineered strain with three biosynthetic pathways To examine the GSH productive capacity of engineered strain W303-1b/FGP in a larger scale, batch cultivation for GSH production was carried out in a 10-L fermentor