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For trypsin digestion, the beads were resuspended in 200 L of 2 M urea in Ca- and Mg-free D-PBS, 2 L of 100 mM calcium chloride (CaCl, Sigma-Aldrich, UK) to achieve a final concentration of 1 mM, and 4 L of 0.5 mg/mL trypsin (2 g total

2023YFC2506100 to WZ), the National Natural Science Foundation of China (81930101 and 82530112 to JXP), the High-level Hospital Construction Project (DFJHBF202102 to WZ), and the Research Foundation of the Guangzhou Municipal Science and Technology Bureau (SL2024A03J01245 to JXP)

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2018;283(5):489-499 Calderon-Larranaga A

Selenate-resistant mutants of Arabidopsis thaliana identify Sultr1;2, a sulfate transporter required for efficient transport of sulfate into roots

doi:10.1089/ars.2006.8.753 11