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10.1016/j.nut.2018.11.015 68
58,60,61 RNA isolation and quantitative RTPCR analysis Total RNA was extracted via TRIzol (Invitrogen), reverse-transcribed into cDNA via a Monad RT Kit, and amplified via SYBR Green Master Mix (Bio-Rad) on a CFX96 Real-Time PCR System

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15 Numerous exciting developments have been made in the past decade, largely from studies of the rod-shaped model organisms Escherichia coli and Bacillus subtilis

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