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By inhibiting NNMT, 5-Amino-1MQ prevents the methylation of nicotinamide, allowing more substrate to enter the NAD+ salvage pathway

Each concentration was tested in triplicate, with each plate also including untreated controls along with a blank cell-free control

NNMT catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to nicotinamide, producing 1-methylnicotinamide (1-MNA)