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BioRad, Hercules, CA, USA) was performed to determine (1) the loss of floxed (recombined) alleles ( loss ) in various brain regions and (2) relative increase of recombined alleles ( gain ) (ctx/cb) using EvaGreen for quantification (Axon, Kaiserslautern, Germany)

Importance of Neurological Symptoms Neurological symptoms are a critical and sometimes early feature of B12 deficiency in PA

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