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All Alexa Fluor secondary antibodies (Life Technologies) were diluted 1:1500 with PBS-Triton X-100, added to the samples for 4 h at 4 C in the dark and subsequently washed 3 times for 20 min in PBS-Triton X-100

doi:10.1172/JCI110155

[DOI] [PMC free article] [PubMed] [Google Scholar] 60.Cordenonsi M, DAtri F, Hammar E, Parry DA, Kendrick-Jones J, Shore D, Citi S