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The slides were conjugated with primary antibodies for 30 min at room temperature, followed by antibody detection using Dako REAL Envision detection system (Agilent Dako, Carpinteria, CA, USA)

10.3390/biomedicines9050526 [DOI] [PMC free article] [PubMed] [Google Scholar] Raghunandan M, Yeo JE, Walter R, Saito K, Harvey AJ, Ittershagen S, Lee E, Yang J, Hoatlin ME, Bielinsky AK, Hendrickson EA, Schrer O, Sobeck A (2020) Functional cross talk between the Fanconi anemia and ATRX/DAXX histone chaperone pathways promotes replication fork recovery

The pEGFP-puro reporter vector was also utilized as a control vector in the transfection procedure

Helps improve elasticity and strengthen the skin barrier

we must not exclude the possibility that the shifts that were observed may not only be due to the antibiotic treatment, but possibly other unknown fitness factors 6

The Skin Biology formula contains approximately five times more GHK-Copper than the strongest formulations available and fifty times the concentration found in most GHK-Copper cosmetics