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Laboratory examination: biochemical blood test, coagulogram, general blood test, hemodynamic parameters

doi: 10.3390/pharmaceutics13122012

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Briefly, about 10 cc of whole blood was collected in a vacutainer ethylenediaminetetraacetic acid (EDTA) tube (Becton Dickinson vacutainer EDTA tube) and spun at 3,000 RPM for 10 mins to separate out plasma, leaving 1 cm of plasma above the buffy coat and taking care not to disturb the buffy coat to circumvent cell contamination