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RNA isolation and real-time quantitative PCR Total RNA was isolated with E.Z.N.A. Total RNA Kit I (Omega Cat#R6834-01) and reverse transcription (RT) reactions were performed with High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Cat#4368814) according to manufacturers protocol

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References Scales CD Jr, Smith AC, Hanley JM, Saigal CS

Under Iriss guidance, Millefiori Aesthetics combines clinical-grade care with a spa-like environment, creating the ideal setting for transformative skincare

[DOI] [PubMed] [Google Scholar] Surmeier D

Jin C, Miners JO, Lillywhite KJ, Mackenzie PI