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For immunoprecipitation, 1 mg of total protein was incubated with 20 L of anti-Flag M2 agarose beads (Sigma-Aldrich) overnight at 4 C with gentle rotation

Indeed, diabetic rat models confirmed that in the liver, the induction of TNF results in increased levels of NF-kB and JNK signaling, characterized by further induction of inducible nitric oxide synthase (iNOS) and consequent increase in nitric oxide production, as well as increased apoptosis rates [71]

three random samples for each experimental condition were used for microarray analysis and all the four samples for quantitative polymerase chain reaction (qPCR) validation

doi: 10.1111/j.1439-0272.2004.00651.x

IRS2-Akt pathway in midbrain dopamine neurons regulates behavioral and cellular responses to opiates

After three 10-min washes in PBS, sections were incubated with the appropriate Alexa Fluor conjugated antibodies for 1 h