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CM5 sensors were first pre-conditioned with the running buffer at a flow rate of 10 L/min for overnight, followed by activation using freshly prepared solution containing 40 mg EDC and 10 mg NHS at a flow rate of 10 L/min for 7 min

HIFs are key transcriptional regulators mediating cellular adaptation to hypoxic environments [484], primarily regulating the expression of a series of oxygen-dependent genes [485]

Acknowledgements The authors benefited greatly from the advice of Jesse Gregory, III, University of Florida, Barry Shane, University of California, Berkeley, and Marian Neuhouser, Fred Hutchinson Cancer Research Center

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Indeed, the addition of FBP significantly reduced complex formation between RCC2 and Rac1 (Fig