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doi: 10.3390/ijms23031445

Thermo Scientific), using a hold of 6% solvent B (80:20 acetonitrile and water, 0.1% formic acid) for 15 min, followed by a gradient of 628% solvent B in 105 min, then 2849% solvent B in 15 min, 40100% solvent B in 10 min, a 10-min hold of 100% solvent B, a return to 5% solvent B in 3 min and finally a 3-min hold of 5% solvent B
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