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For passaging of organoids, the collagen domes were incubated with 1 mg ml 1 collagenase from Clostridium histolyticum (Sigma-Aldrich) for 30 min at 37 C, and digestion monitored using bright-field microscopy (small clumps of cells)

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Health Quality Ontarios research is published as part of the Ontario Health Technology Assessment Series, which is indexed in MEDLINE/PubMed, Excerpta Medica/Embase, and the Centre for Reviews and Dissemination database

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