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Then, they were homogenized with TRIzol reagent (Invitrogen, UK) by Tissue Lyser LT (Qiagen-Izasa, Spain) and RNA was extracted and further purified using the QIAGEN RNeasy Mini Kit including digestion with RNase-free DNase, following the manufacturers instructions

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Sglutathionylation can proceed via interaction between PrSH or GSH and oxidized derivative of protein cysteine residue, e.g., sulfenic acid (SOH), thiyl radical (S ), Snitrosyl (SNO), thiosulfinate (-S(O)SR), or sulfenyl amide (cyclicSNCO) (Fig
