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Compound 2 was added to the solution of ( N - tert -butoxycarbonyl)-L-selenocysteine methyl ester in either methanol or phosphate buffer and incubated for 40 min, 24 h, and 120 h under N 2 gas balloon

Additionally, like other oxidases nsHb is also capable of catalyzing protein nitration through a nitrite- and hydrogen peroxide-dependent process

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Compound 3 was isolated by silica gel column chromatography using a dichloromethane/methanol (DCM:MeOH = 40:1, V/V) eluent (yield: 75%)