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Samples were prepared by mixing volumes of desired protein and ligand such that the final protein concentration in each case is 5 M, C 55 -P at 20 M, lipid I at 5 M, lipid II at 5 M

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The model mice were given engineered probiotics prior to being exposed to a virulent strain of C

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Pyridinium derivatives have since been used in many protocols, although additional matrices are sometimes required to achieve sufficiently high ionization efficiency