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The intensity of the resulting MDA-TBA adduct was read at RFU at Ex/Em = 532/553 nm and the data were expressed as nmol/mg protein

If a second primary antibody was used, the cells were incubated either for 2 h at room temperature or overnight at 4C

We centrifuged the samples (3000 rpm, 5 min), and collected the supernatants

10.1016/j.theriogenology.2008.10.002 80

GHK-Cu has been shown to accelerate wound closure, reduce scarring, and promote the formation of new blood vessels (angiogenesis) crucial for tissue repair

Therefore, they require external antioxidant support in the form of oral glutathione supplements in Pakistan, like Gluvit-S Glutathione Oral Liquid