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The griffithsin dimer is required for high-potency inhibition of HIV-1: evidence for manipulation of the structure of gp120 as part of the griffithsin dimer mechanism

HED/Cys-SG and CHP assays If not otherwise indicated, strep-MBP-GRX samples were reduced with 10 mM DTT for 2 hours and the buffer was exchanged against 100 mM Tris/HCl p H 8.0, 1 mM EDTA 15 using PD MiniTrap G-25 desalting columns (GE Healthcare)

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