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S27), compared to the literature value of 1300 M -1 s -1 for the related reaction of phenyl maleimide 50

The pET32 (Novagen), one of the commercially available vectors for Trx tagging, carries this dual-fusion partners for protein production and purification ( Trx fusion partner can also be useful in protein crystallization of certain target proteins because it readily forms several crystals itself, and it offers a rigid connection to the target protein, which is an essential feature for blocking conformational heterogeneity usually found in various attempts of fusion proteins crystallization (Smyth et al., 2003

Drug Targets 12 , 168178 (2012)

SemD binds simultaneously to several host partners Our findings thus far indicate that SemD contains spatially separated binding sites that are connected by highly flexible linker regions, which sterically allow simultaneous interactions with the PM, N-WASP and SNX9

[DOI] [PubMed] [Google Scholar] Lan T, Yang ZL, Yang X, Liu YJ, Wang XR, Zeng QY