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reported the TCM decoction Bu Shen Hua Zhuo formula (BSHZF) administration improved gut microbiota function in rats with letrozole-induced PCOS and inhibited the activation of the TLR4/NF-kB signaling pathway in PCOS-related ovarian tissue, decreasing the pro-inflammatory cytokines TNF-a, IL-6, and IL-8 (236)

doi: 10.3389/fphar.2019.00250 Received 24 October 2018 Accepted 26 February 2019 Published 20 March 2019 Volume 10 - 2019 Edited by Annette Nicke, Ludwig Maximilian University of Munich, Germany Reviewed by Hai Minh Nguyen, University of California, Davis, United States

Interestingly, in the PREvencin con DIeta MEDiterrnea-Plus (Predimed-Plus) trial in overweight or obese individuals with metabolic syndrome, an increase in dietary choline intake over a one-year period was associated with improved measures of cardiovascular health (i.e., lower serum total cholesterol, serum LDL cholesterol, and systolic and diastolic blood pressure levels) (123)

doi: 10.1016/j.ijpharm.2014.07.025 [DOI] [PubMed] [Google Scholar] 99.Bonaccorso A, Musumeci T, Serapide MF, Pellitteri R, Uchegbu IF, Puglisi G

Apart from low folate absorption levels related to riboflavin-deficiency-induced gut inflammation (49), riboflavin is required for biosynthesis of folate coenzymes without which lower levels of useful folate are found in animal studies (50)