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Furthermore, as an iron chelator, gallic acids iron-chelating ability itself may also contribute to ferroptosis inhibition (Strlic et al., 2002)

Mice tissues were resected and fixed with 4% paraformaldehyde (Leagene Biotechnology, China), and immunohistochemistry staining was conducted according to our previous publication [44]

Samples were processed in alcohol and xylol using the PT05 TS tissue processor (LUPETEC, UK) and embedded in histological paraffin (Histosec, Sigma)

The concentration in the supernatant was determined utilizing a standard curve ranging from 4250 pg/mL and 12750 pg/mL for IL-1 and IL-18, respectively

IEC-6 cells were allocated into five experimental groups: (i) Control (CTL), cultured in serum-free DMEM for 12 h

Henceforth, the expression of stable yet divergent gene regulatory networks can be assigned to specify locations without being negated due to the dilution effect of direct unsupervised analysis
