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How to Use Creatine Loading Phase (Optional): Some people do a loading phase by taking 20 grams of creatine a day (4 doses of 5 g each) for 5 to 7 days
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Cells were dehydrated with an ethanol series (20%, 50%, 70%, 90% and 100%) on ice for 1 min each and then 100% dry ethanol at room temperature three times for 1 min each, infiltrated with 50:50 dry ethanol:Durcupan ACM resin (Sigma-Aldrich, 44610) for 30 min and then four changes of Durcupan at 1 h each and finally embedded in a vacuum oven at 60 C for 72 h
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