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Excess iron plays a vital role in the generation of harmful oxygen radicals due to its catalysis of one electron redox chemistry

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All real-time PCRs were performed three times and all changes in gene expression were reported as multiples of increases relative to the control while GAPDH was used as an internal control and relative gene expression between the experimental groups were determined using MxPro software

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The studys protocol was accepted by the Biomedical Ethics Research Council, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia (16819)

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