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The His6-SUMO tag was cleaved by ULP1 treatment, and the tag-free protein was fractioned with Ni-NTA column and Superdex 75size exclusion column (GE Healthcare) with 25 mM HEPES (pH 8.0), 300 mM NaCl, and 1% glycerol
New dawn for atherosclerosis: vascular endothelial cell senescence and death

Organoid models of human liver cancers derived from tumor needle biopsies

The initial inflammatory phase is characterized by platelet activation, the recruitment of immune cells, the release of growth factors and cytokine, followed by the secretion of growth factors and enhanced cell proliferation, and finally, in a remodeling phase, collagen production and histogenesis leads to mature scars (125)

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