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Isolated cells were then plated onto glass coverslips (5 10 4 cells/cm 2 ) coated with 0.1 mg/mL poly-L-lysine (Sigma) and 2 h after seeding, the plating medium was substituted by complete growth medium (Neurobasal medium supplemented with 2% B27 and 2 mM Glutamax)

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Regulation of xCT expression and system x (c) (-) function in neuronal cells

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